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304-2호 Confocal Microscope
 
모델명 : LSM710
제조회사 : Carl Zeiss
도입시기 : 2011. 06
담당자 : 김은영 aualright@naver.com
위치 : 304-2호
전화번호 : 2049-6001
사용요율 :
 
 
Confocal Laser Scanning Microscope는 광축에 Pinhole을 설치하여 Specimen을 통과 또는 Specimen에서 반사되는 광선 중 초점이 정확하게 맞는 광선만을 선택할 수 있도록 함으로써 해상력을 극대화시켰고 현미경의 대안렌즈를 통해 세포 및 시료를 구별하게 관찰하면서 영상을 얻을 수 있는 장비
1) Scanners : two independent, galvanometric scan mirrors with ultra-short line and frame flyback
2) Scan Zoom :0.6x to 40x; digital variable in steps of 0.1(on Axio Examiner 0.67x to 40x)
3) Scanning speed : 14 x 2 speed stages; up to 12.5 frames/sec with 256 x 256pixels;5 frames/sec
4) Detectors : Standard 2 confocal fluorescence channels with highly sensitive low dark noise PMTs
5) Lasers: 1) Ar-laser (458, 488, 514nm) 25mW
2) HeNe-laser (543nm) 1mW
3) HeNe-laser (633nm) 5mW (pre-fiber manufacturer specification)
6) Antivibration table - Active air damped, 900x750x770mm (LxDxH)
7) Software (ZEN)
System configuration
o Comfortable configuration of all motorized of the scanning module, the lasers and the microscope
o Saving and restoring of application-specific configurations (ReUse)
System self-test
o Calibration and testing tool for the automatic verification and optimal adjustment of the system
Acquisition modes, Smart setup
o Spot, line/spline, frame, z-stack, lambda stack, time series and all combinations(xyz λt)
o Step scan (for higher frame rates)
o Smart acquisition setup by selection of dyes
8) Objective : 10x, 20x, 40x


· 3D examinations
· Multifluorescence
· Colocalization
· Ion imaging
· FRET (various methods)